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网友留言-CCCTCTAGAAATAATTTTGTTTAACTTTAAGAA Sense strand PCR primer for your building of pET-pfJRTS232--纽带成品网站超市-专业建站99元全包-送域名-免费招代理-纽带建站超市
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发布于:2019-8-12 19:50:49  访问:66 次 回复: 篇
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CCCTCTAGAAATAATTTTGTTTAACTTTAAGAA Sense strand PCR primer for your building of pET-pfJRTS232-
Underlined sequence will be the restriction web-site for XbaI Sense strand PCR primer to organize construct pET-pfJRTS232-299. Bold-face letters represent Fulfilled released before Glu300. Mitoglitazone Insulin Receptor PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/19444300 Underlined sequence is PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/21411495 the restriction web page for NcoI Antisense strand PCR primer with the development of all pET-pfJRTS mutants. Underlined sequence would be the restriction web-site for BamHIJRTS232-JRTS232-N3TSACTCATGGATCCTTAAGCAGCCTable 2 mce medchemexpress Primers employed for development of truncated bifunctional pfDHFR-TSName 229-277 Perception 229-277 Antisense 229-276 Perception 229-276 Antisense 229-275 Perception 229-275 Antisense 229-274 Perception 229-274 Antisense 229-271 Sense 229-271 Antisense 229-265 Feeling 229-265 Antisense Size (bases) 60 sixty sixty 60 sixty sixty 60 60 sixty sixty sixty 60 Sequence (five 3) ACAACATTGGATTTTATCATTTATAAGAAATAT GAAAATGATGATGATGATGAAGAAGAA TTCTTCTTCATCATCATCATCATTTTCATATTTC TTATAAATGATAAAATCCAATGTTGT ACAACATTGGATTTTATCATTTATAAGAAAAAT TATGAAAATGATGATGATGATGAAGAA TTCTTCATCATCATCATCATTTTCATAATTTTTC TTATAAATGATAAAATCCAATGTTGT ACAACATTGGATTTTATCATTTATAAGAAA ATTAATTATGAAAATGATGATGATGATGAA TTCATCATCATCATCATTTTCATAATTAATTTTC TTATAAATGATAAAATCCAATGTTGT ACAACATTGGATTTTATCATTTATAAGAAAAAA ATTAATTATGAAAATGATGATGATGAT ATCATCATCATCATTTTCATAATTAATTTTTTTCT TATAAATGATAAAATCCAATGTTGT ACAACATTGGATTTTATCATTTATAAGAAAGAC AAATATAAAATTAATTATGAAAATGAT ATCATTTTCATAATTAATTTTATATTTGTCTTTCTT ATAAATGATAAAATCCAATGTTGT ACAACATTGGATTTTATCATTTATAAGAAAGAA TTTTACAAAAATGTAGACAAATATAAA TTTATATTTGTCTACATTTTTGTAAAATTCTTTCT TATAAATGATAAAATCCAATGTTGT Utilities and description Perception strand PCR primer to the design of pfDHFR-TS229277. Lys228 (italic) was accompanied by Tyr278(daring) Antisense strand PCR primer for your development of pfDHFRTS229-277 Perception strand PCR primer with the construction of pfDHFR-TS229276. Lys228 (italic) was followed by Asn277(daring) Antisense strand PCR primer to amplify pfDHFR-TS229-276 Perception strand PCR primer to the building of pfDHFR-TS229275. Lys228 (italic) was followed by Ile276(bold) Antisense strand PCR primer to amplify pfDHFR-TS229-275 Perception strand PCR primer for the building of pfDHFR-TS229274. Lys228 (italic) was followed by Lys275(bold) Antisense strand PCR primer to amplify pfDHFR-TS229-274 Perception strand PCR primer for that building of pfDHFR-TS229271. Lys228 (italic) was followed by Asp272(daring) Antisense strand PCR primer to amplify pfDHFR-TS229-271 Feeling strand PCR primer to the development of pfDHFR-TS229265. Lys228 (italic) was followed by Glu266(bold) Antisense strand PCR primer to amplify pfDHFR-TS229-Chaianantakul et al. Malaria Journal 2013, 12:91 http://www.malariajournal.com/content/12/1/Page 4 ofto yield plasmids pET-pfJRTS232-235, pET-pfJRTS232251, pET-pfJRTS232-265, pET-pfJRTS232-271, pETpfJRTS232-274, pET-pfJRTS232-276, pET-pfJRTS 232-277, and pET-pfJRTS232-299. These mutant pETpfJRTS plasmids ended up reworked into electro-competent E. coli strain 2913 cells by electroporation employing pulses established at one.8 kV, 400, 25 F and a pulse size of 810 min. Just after centrifugation at six,500 g at 4 for 10 min and resuspending th.CCCTCTAGAAATAATTTTGTTTAACTTTAAGAA Sense strand PCR primer for the building of pET-pfJRTS232-276. GGAGATATACCATGAATTATGAA Bold-face letters depict Met introduced in front of Asn277. Underlined sequence will be the restriction website for XbaI CCCCTCTAGAAATAATTTTGTTTAACTTTAA GAAGGAGATATACCATGTATGAAAAT AAAGAATCCCATGGAAGAGAAAAATAAA Sense strand PCR primer to get ready construct pET-pfJRTS232-277. Bold-face letters characterize Fulfilled launched in front of Tyr278. Underlined sequence would be the restriction web site for XbaI Sense strand PCR primer to organize assemble pET-pfJRTS232-299. Bold-face letters symbolize Satisfied introduced in front of Glu300. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/19444300 Underlined sequence is PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/21411495 the restriction internet site for NcoI Antisense strand PCR primer for that construction of all pET-pfJRTS mutants.
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